■ 基本信息

■ 質粒屬性

■ 質粒簡介

pFastBac Dual昆蟲細胞表達載體包含有兩個啟動子，能夠同時表達兩個外源基因，一個表達基因是polyhedrin (PH)啟動子控制，另一個外源基因是由p10啟動子控制。

pFastBacDual 載體的特點是在單個載體上有兩個啟動子，用于在使用 Bac-to-Bac? 桿狀病毒表達系統(Bac-to-Bac? Baculovirus Expression System) 時在昆蟲細胞中同時表達兩個蛋白質。該載體具有兩個強啟動子，多角體蛋白啟動子和p10 啟動子，因此可實現高水平表達。包含 CAT和β-葡萄糖醛酸酶 (gus) 的表達對照。

The Bac-to-Bac? Vector Kit contains a pFastBac? 1 vector, as well an expression control vector, intended for use as part of the Bac-to-Bac? Baculovirus Expression System , which enables the efficent production of recombinant baculovirus for expression testing in insect cells. The Bac-to-Bac? System relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells (Figure 1). This system features:

Time-saving expression bacmid. With Bac-to-Bac?, the expression cassette of the pFastBac? vector recombines with the parent bacmid in DH10Bac? E. coli Competent Cells (not included with this vector kit) to form an expression bacmid. The bacmid is then transfected into insect cells for production of recombinant baculovirus particles.

Easy colony screening. The parent bacmid in DH10Bac? E. colicontains a segment of the lacZα gene. The lacZα gene is disrupted upon transposition of the expression cassette into the bacmid allowing for blue/white selection of recombinants. This makes identification of recombinant colonies easy.

■ 質粒圖譜

■ 質粒序列

質粒序列請下載：ZK1045pFastBacDual昆蟲雙框質粒.txt

質粒只保證關鍵序列正確，不保證表達效果。