■ 基本信息
啟動子: | T7 |
復制子: | M13 |
質粒分類: | 大腸桿菌載體；蛋白表達質粒 |
質粒大小: | 7477bp |
質粒標簽: | Intein |
原核抗性: | Amp |
克隆菌株: | DH5a |
培養條件: | 37℃，有氧，LB |
表達宿主: | 大腸桿菌 |
5'測序引物: | T7:TAATACGACTCACTATAGGG |
3'測序引物: | 根據序列設計 |
備注: | 表達質粒 |
■ 質粒屬性
質粒宿主: | 大腸桿菌 |
質粒用途: | 蛋白表達 |
片段類型: | ORF |
片段物種: | 空載體 |
原核抗性: | Amp |
真核抗性: |
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熒光標記: |
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■ 質粒簡介
The pTYB1 is an E. coli vector created for fusion protein expression and purification. A target gene can be inserted into the the ORF by use of the MCS upstream of the Sce VMA intein. The NdeI site in the MCS has the initiator sequence ATG that enables translation of the ORF. The chitin-binding domain(CBD) downstream of the intein results in a target protein-intein with C-terminal chitin beads that facilitate protein purification. Release of the protein is induced by the thiol cleavage activity of the intein.
質粒只保證關鍵序列正確，不保證表達效果。
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK335pTYB1大腸表達質粒.txt
