■ 基本信息
啟動子: | TRE3G |
復制子: | pUC |
終止子: | SV40 poly(A) signal |
質粒分類: | 病毒系列，慢病毒克隆載體 |
質粒大小: | 9100bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | Stbl3 |
培養條件: | 37度 |
表達宿主: | 哺乳細胞 |
誘導方式: | 四環素誘導 |
5'測序引物:
| M13R:CAGGAAACAGCTATGACC
|
3'測序引物: | 根據序列設計引物 |
■ 質粒屬性
質粒宿主: | 哺乳細胞,慢病毒 |
質粒用途: | 蛋白表達 |
片段類型: | ORF
|
片段物種: | 空載體
|
原核抗性: | Amp |
真核抗性: | Puro
|
熒光標記: | 綠色
|
■ 質粒簡介
The inducible promoter PTRE3G provides for very low basal expression and high maximal expression after induction. It consists of 7 repeats of a 19 bp tet operator sequence located upstream of a minimal CMV promoter. PTRE3GV is a version of PTRE3G that was modified at Clontech for higher performance in lentiviruses and retroviruses. In the presence of Dox, Tet-On 3G binds specifically to PTRE3GV and activates transcription of the downstream GOI. PTRE3GV lacks binding sites for endogenous mammalian transcription factors, so it is virtually silent in the absence of induction. pLVX-TRE3G-ZsGreen1 is an HIV-1-based, inducible lentiviral expression vector that allows the simultaneous expression of ZsGreen1 and your protein of interest in virtually any mammalian cell type, including primary cells.
■ 質粒圖譜
■ 質粒序列
質粒序列請下載：
ZK774pLVX-TRE3G-Zsgreen1慢病毒表達質粒.txt
